| dc.description.abstract | Sixty luve blades representing 14 different plant species in the Woods Hole, Massachusetts, area were collected for the irdatlon of phyllopl.ne bacteria employing a leaf-print method on 10% King's B medium supplemented with 100 pghl cyclohesimldc. Colonies that appeared after 2 3 days were overlaid with Laria Agar (pH 7.4) containing approximately 10' cdlslml of BckaicMo cod carrying Vibriofirckeri hu operon with a 250-base pair non-polar deletkm hr W. Presumptive autoinducer-producing bacteria were isolated from the spots of biolnminescens in the overlaid plates. These kinds of phylloplme bacteria represented approximately less than two percent of the total Ieaf-sulrce bacteria Based on preliminary observation of cdony morphology and limited physiologkd assays, they were two main groups of phylloplane bacteria belonltiag to either Xuntkmona sp. or A b n 4 ~q1vh gw, Accenlda(lty, thb method bas the potential to be developed into a rapid and reliable diqphostic test, to study the distribution u well u relative abundmre of certain strains or p a t h n of phytloplane bacteria. | id |
