The Effectivity of Indol-3-Butyric Acid (IBA), 6-Benzyl Amino Purin (BAP), and Sucrose to Improve In Vitro Multiplication of Temulawak (Curcuma xanthorrizha Roxb.)

Abstract

Temulawak is one of the most important medicinal plant. Superior propagule is needed to improve productivity and quality of temulawak. Tissue culture or in vitro culture is an alternative way to produce superior and healty propagule. Application of plant growth regulator and energy source are very important for in vitro plant regeneration and growth. The objective of this reseach has been to study the effect of IBA, BAP and sucrose consentrations added to MS medium on in vitro regeneration of temulawak. This research consists of two factorial experiments. The first experiment was to study the effect of IBA (0, 0.25, 0.5, 0.75 mg/L) and BAP (0, 1, 2 ,3 mg/L) . The second experiment was to study the effect of BAP (0, 1, 2 and 3 mg/L) and surose (30, 40, 50 and 60 g/L). Experiments were arranged in Randomize of Completely Block Design. The result of first experiment showed that combination of IBA 0. 75 mg/L with/without BAP gave the lowest shoot number, while appllication of 2 or 3 mg/L BAP with 0.0, 0.25 or 0.50 mg/L IBA can produce highest number of shoot. There were interaction between IBA dan BAP concentration on the number of shoot, leaves, and plantlet height. On the second experiment, result showed that BAP significantly increased the number of shoot but there was no significant different between BAP 1, 2 and 3 mg/L. BAP more than 1.0 mg/L reduced plantlet height, while sucrose at 40 g/L gave the highest plantlet height.