Chitosan Protection To Saga Leaves Extract (Abrus Precatorius Linn) And Lingzhi Mushroom (Ganoderma Lucidum) From Rumen Microbial Degradation
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The purpose of research was study the use of 2% chitosan as a protector of herb saga leaves extract (Abrus precatorius Linn) and lingzhi mushroom (Ganoderma lucidum) from microbial degradation in the rumen in vitro. Randomized block design with five ration treatments and three times collection of rumen fluid as a block. The treatments were R0 (control) = basal ration (35% forage and 65% concentrates), R1: R0+ saga leaves extract, and R2: R0+ saga leaves extract protected by 2% chitosan, R3: R0+ lingzhi mushroom extract, and R4: R0+ lingzhi mushroom extract protected by 2% chitosan. Parameters observed were total population of rumen microbes (protozoa and bacteria), concentrations of total VFA and NH3, dry and organic matter digestibility (DMD and OMD). The results showed chitosan protection to both extracts were not influence the population of bacteria and protozoa as well as NH3 concentration, but decreased 16,4% VFA total (P<0.05) and 35% average digestibility (P<0.05) in saga leaves extract and also decreased 20% VFA total (P<0.05) in lingzhi mushroom extract without interfere the digestibility. As conclusion, 2% chitosan can be used as protector to lingzhi mushroom extract than to saga leaves extract from degradation of rumen microbes due to its ability maintaining the digestibility.