| dc.description.abstract | Summary
Nitrosamines and malonaldehyde (MDA), the toxic and mutagenic compound
are could be formed in dendeng. But their formation are affected by sodium
nitrate/nitrite that used in curing process and ingredients that are rich in antioxidant
component. The research before resulted the best formulation that combines spices
composition, wet curing and preparation technique that produce good characteristics of
dendeng, especially in red colour, and the lowest MDA. That dendeng has no nitrite
residual detected by spectrophotometer, but has probability of nitrosamine containing
under limit of detection of GC-MS that was used (<26,85 µg/kg of NDEA). Therefore it
is necessary to evaluate the consumption safety of dendeng affected by MDA and
nitrosamine that are probably present in that product.
The objective of this research was to evaluate the safety consumption of beef
dendeng used rats as animal models fed ration containing dendeng as proteins source
for 42 days that was combined with stress oxidative treatment by interaperitoneal
injection of N-nitrosodiethylamine (NDEA). Rats were devided into 5 group of
treatment:
1. group fed ration containing freeze dried meat flour as source of proteins without
NDEA injection (control group);
2. group fed ration containing non-cured dendeng as source of proteins, without
NDEA injection (C0TN);
3. group fed ration containing non-cured dendeng as source of proteins, with NDEA
injection (C0N);
4. group fed ration containing cured dendeng as source of proteins, without NDEA
injection (CbTN);
5. group fed ration containing cured dendeng as source of proteins, with NDEA
injection (CbN) .
Evaluating was done by monitoring of animal performance, measuring of kidney and
liver weight, analysis of MDA (plasma, kidney, and liver), analysis of katalase and SOD
activities (kidney and liver). Beside that general morphology evaluation was done on
histologis of kidney, liver and pankreas using hematoksilin-eosin staining, also SOD
status in kidneys and liver were analysed as confirmation used immunohistochemical
staining technique .
The result showed that either cured dendeng, or non-cured dendeng did not
affect kidneys or liver weight, with the ration conversion were not different to control
ration. Dendeng consumption did not increase MDA level compared to control
treatment, indeed increased kidneys and liver katalase and SOD activities. Cured
dendeng affected degeneration cellular of kidneys and liver like cellular injury caused
by injection of NDEA. As the conclusion, dendeng modified is safe to use as animal
proteins source, but for cured dendeng still has risk on kidneys and liver degenerative
diseases if it was consumed continuously for long periods.
Keywords: beef dendeng safety, nitrite, nitrosamine, malonaldehyde | id |
