Polymorphisms of Growth Hormone (GH|MspI) Gene in Indonesia Local Chicken and the Crossbred using PCR-RFLP

Abstract

The aimed of the research was to identify the chicken growth hormone (cGH) gene polymorphisms in local chicken, the crossbred, commercial meat type and layer. cGH gene plays a crucial role in controlling growth and metabolism, leading to potential correlation between cGH gene polymorphisms and economic traits such as meat and egg production. Polymorphism in intron 4 of cGH gene was studied in 229 chicken blood samples consist of kampung, pelung, sentul, commercial meat type, layer, merawang, commercial meat type x kampung, kampung x commercial meat type, sentul x kampung and pelung x sentul chickens. A specific primer set was used to amplify a fragment of growth hormone locus using PCR. PCR products were digested with MspIrestriction endonucleases. The amplified fragment digested with MspIenzyme revealed 2 alleles A and B with the frequency of 0.88-0.96 and 0.04-0.12, except in layer chicken only had 1 allele A with the frequency of 1.00. Results showed that cGH gene in MspI locus at kampung, pelung, sentul, commercial meat type, merawang, commercial meat type x kampung, kampung x commercial meat type, sentul x kampung and pelung x sentul chickens were polymorphics, except the layer chicken was monomorphics. The populations of Indonesia local chicken that was studied in this research had low heterozygosity but it was still in the Hardy-Weinberg equilibrium.