Actinophytocola timorensis sp. nov. and Actinophytoco/a corallina sp. nov., isolated from soil

Abstract

Two actinomycete strains, ID05-A0653T and ID06-A0464r, were isolated from soils of West Timor and Lombok island, respectively, in Indonesia 16S rRNA gene sequence analysis clearly demonstrated that the isolates belonged to the family Psaudonocardiaceaa and were closely related to the genus Actinophytocola. Strains 1005-A0653 T and ID06-A0464 T exhibited 98.1 and 98.2 % 16S rRNA gene sequence similarity, respectively, with Actinophytocola oryzae GMKU 367T. The isolates grew well on ISP media and produced white aerial mycelium. Short spore chains were formed directly on the substrate mycelium. The isolates contained mesodiaminopimelic acid, arabinose and galactose as cell-wall components, MK-9(H4) as the sole isoprenoid quinone, iso-C16:o as the major cellular fatty acid and phosphatidylethanolamine as the diagnostic polar lipid. The DNA G + C contents of strains ID05-A0653 T and ID06-A0464 T were 69. 7 and 71.2 mot%, respectively. On the basis of phenotypic characteristics, DNA-DNA relatedness and 16S rRNA gene sequence comparisons, strains ID05-A0653 T and 1006-A0464 T each represent a novel species of the genus Actinophytoco/a, for which the names Actinophytocola timorensis sp. nov. (type strain ID05-A0653T =BTCC B-673T =NBRC 105524T) and Actinophytcx;ola corallina sp. nov. (type strain ID06-A0464T =BTCC B-674T =NBRC 105525T) are proposed.