Identification of Aroma Gene (Mutated badh2) and Properties of Aroma on Aromatic BC5F 2 Ciherang

Abstract

Aromatic rice varieties have some weaknesses such as low productivity, and less resistant to pests and diseases. This study aimed to obtain homo:::ygous strain ofBCF'2 Ciherang aromatic through the identification of aroma gene (mutated badh2) and properties of the aroma. Ciherangpaddy (nonaromatic paddy) was used as the female parent, whereas Mentik Wangi paddy (aromatic paddy) was used as the male parent. The experiment was conducted in BCJ2 because it is expected to generate plants with properties 98. 4% close to female parent. The DNA from five strains of paddy plants BC J 2 Ciherang X }vfentik Wangi was isolated by a modified CTAB melhod. The concenlralion of DNA was determined by measuring absorbance at 260 nm wavelength, while its purity was determined from the ratio of the absorbance at a wavelength of2601280 nm. PCR-based molecular selection was done by using the Bradbury primers. PCR results showed that of the 250 samples, there were 66 samples had DNA.fragment of the same size as that of Mentik Wangi, i.e. 257 bp, 67 samples had the same size as the DNAfragment ofCiherang, i.e. 355 bp, and 117 samples had the same size with the both of DNA fragments, i.e. 257 bp and 355 bp. Plants with amplified 257 bp DNA fragment was subjected to leaf aroma test using 1. 7% KOH. The results showed that 42 positive samples, out of 66 samples. Samples positive on leaf aroma test were tested again on rice aroma test. Rice aroma test results showed the majority (85.4%) samples that are positive on leaf aroma lest is also positive on the rice aroma lesl.