Pengembangan Formulasi Biopestisida Berbahan Aktif Bakteri Staphylococcus epidermidis dan Bacillus subtilis untuk Mengendalikan Penyakit Layu Bakteri oleh Ralstonia solanacearum pada Tomat

Abstract

Application of biocontrol agents in the field often failed because of some factors, one of them was caused by the decreasing of the population of the biocontrol agents and their activities during storage or transportation from the laboratories. In order to minimize that problem, formulations were developed using medium that able to support the viability and maintain their characters during storage. This research was conducted to evaluate the effects of liquid and powder formula to the viability of the endophytic bacteria (Staphylococcus epidermidis BC4) and PGPR (Bacillus subtilis AB89) and their effectiveness to control the bacterial wilt disease of tomato. Viability of the bacteria in the formula was counted from the first week up to 8th weeks of storage using plate-count method. The effectiveness of the formula to the bacterial wilt disease was evaluated in the green house. Population of S. epidermidis in the liquid and powder formula was still stable up to 8 weeks of storage, i.e. 3.0 x 108 cfu/ml and 2.3 x 108 cfu/g. Population of B. subtilis both in liquid or powder formula was decreasing during storage. The Area Under Disease Progress Curve (AUDPC) value of the bacterial wilt disease incidence on the plants treated with S. epidermidis or B. subtilis in liquid formula was significantly lower compared with control. All of the formulas were not inhibit the height of tomato plants