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      Aplikasi metode medan listrik tegangan tinggi (high pulsed Electric field) sebagai cara mempertahankan kualitas Fisik, kimia dan mikrobiologis susu segar

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      Date
      2009
      Author
      Maheswari, Rarah Ratih Adjie
      Sutrisno
      Bakar, Abu
      Hariono, Budi
      Stefani, Ida Ayu Ratih
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      Abstract
      Aplikasi Medan Pulsa Listrik Tegangan Tinggi (HPEF) didasarkan pada dua teori utama, yaitu electrical breakdown dan teori elektroporasi membran sel akibat adanya medan listrik tegangan tinggi yang mengakibatkan inaktivasi sel. Inaktivasi mikroba susu utuh pada suhu kamar (27±1oC) lebih baik dibandingkan inaktivasi pada suhu dingin (4-8oC) untuk jarak elektrode 3 mm; 4 mm; 5 mm dengan chamber tipe pararel plate sistem bacth berturut-turut adalah 26,16 % dan 11,34 %; 19,28 % dan 3,65 % dan 8,11 % dan 0,36 %. Inaktivasi bakteri pathogen E. coli; Staphylococcus aureus dan Salmonella Typhimurium jika dibandingkan dengan jumlah mikroba pada suhu kamar dengan sumber pembangkitan coil 30 kV untuk jarak elektrode 3 mm berturut-turut adalah 1,085 log siklus; 0,53584 log siklus dan 0,908 log siklus. Koefisen (μ) laju inaktivasi bakteri pathogen E. coli; Staphylococcus aureus dan Salmonella Typhimurium berturut-turut adalah 0,0194; 0,0112 dan 0,0063 . Penelitian pengujian kualitas susu metode HPEF memberikan hasil sebagai berikut : (1) BJ SNI, susu segar dan susu metode HPEF dengan waktu 30” dan 60” berturut-turut adalah 1,0280; 1,026; 1,0235; dan 1,0235 g/cm3 (2) kadar lemak SNI, susu segar dan susu metode HPEF 30” dan 60” berturut-turut adalah 2,8 %; 3,215 %; 2,925 %; dan 2,87 %, (3) SNF SNI, susu segar dan susu pasteurisasi 30” dan 60” berturut-turut adalah 7,7 %; 7,91 %; 7,28 %; dan 7,24 % (4) kandungan protein SNI, susu segar dan susu pasteurisasi 30” dan 60” berturut-turut adalah 2,5 %; 3,21 %; 2,99 % dan 2,97 %.
       
      Applications of High Pulsed Electric Field (HPEF) is based on two main theories, namely the theory of electrical breakdown and electroporation membrane cell due to high pulsed electric field resulting in cell inactivation. Microbial inactivation whole milk at room temperature (27 ± 1oC) better than the inactivation at low temperature (4-8oC) gap electrode: 3 mm; 4 mm, 5 mm with a parallel plate type systems bacth chamber, respectively 26.16% and 11.34%; 19.28% and 3.65% and 8.11% and 0.36%. Inactivation bacterial pathogen of E. coli; Staphylococcus aureus and Salmonella Typhimurium when compared to the number of microbes at room temperature with the coil 30 kV with gap electrode 3 mm, respectively was 1.085 log cycles; 0.53584 log cycle and 0.908 log cycles. Coefficient (μ) rate inactivation bacterial pathogen of E. coli; Staphylococcus aureus and Salmonella Typhimurium, respectively 0.0194; 0.0112 and 0.0063. Testing milk quality HPEF methods give results as follows: (1) BJ SNI, fresh milk and milk HPEF method with a 30 "and 60" respectively is 1.0280; 1.026; 1.0235, and 1.0235 g / cm3 (2) SNI fat, fresh milk and milk methods HPEF 30 "and 60" are respectively 2.8%; 3.215%; 2.925% and 2.87%, (3) SNF SNI, fresh milk and milk pasteurization 30 "and 60" respectively 7.7%; 7.91%; 7.28% and 7.24% (4) the protein content of SNI, fresh milk and milk pasteurization 30 "and 60" respectively was 2.5%; 3.21%; 2.99% and 2.97%
       
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      http://repository.ipb.ac.id/handle/123456789/71690
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