Browsing Anatomy, Phisiology and Pharmacology by Author "Takagi, M."
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Direct rehydration of in vitro fertilised bovine embryos after vitrification
Saha, S | Takagi, M. | Boediono, Arief | Suzuki, Tatsuyuki (1994)VITRIFICATION is a valuable embryo freezing technique because it is simple, quick, economic (Rall and Fahy 1985), reli able and reasonably easy to apply under field conditions (Niemann 1991). ... -
Fertilization and Development of Frozen–Thawed Germinal Vesicle Bovine Oocytes by a One-Step Dilution Method in Vitro
Suzuki, T. | Boediono, Arief | Takagi, M. | Saha, S. | Sumantri, Cece (1996)The objective of this study was to evaluate in vitro fertilization and cleavage rates of frozen–thawed bovine oocytes at the germinal vesicle (GV) stage. In mouse oocytes, spindle microtubule reorganization after GV breakdown ... -
Influence of day-0 and day-7 superovulated cow serum during development of bovine oocytes in vitro
Boediono, Arief | Takagi, M. | Saha, S. | Suzuki, Tatsuyuki (1994)Oocytes were matured in medium supplemented with 5% serum collected from superovulated cows at oestrus (Day-0 SCS) or at the time of embryo collection (Day-7 SCS), or in medium supplemented with fetal calf serum (FCS). ... -
The influence of polyvinylpyrrolidone on freezing of bovine IVF blastocysts following biopsy
Suzuki, Tatsuyuki | Saha, S. | Sumantri, Cece | Takagi, M. | Boediono, Arief (1995)A study was conducted to develop a better freezing protocol for in vitro developed biopsled bovine blastocysts. Biopsied blastocysts were exposed to 1.8 M ethylene glycol (EG) + 0.05 M trehalose (T) and different their in ...