Browsing Anatomy, Phisiology and Pharmacology by Author "Sumantri, Cece"
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Chimera production in bovine and its sperm capacity
Boediono, Arief | Sumantri, Cece | Suzuki, T. (1998)In vitro produced bovine embryos were used to produce chimeras by combining the 8- cell stage embryos. Aggregated embryos were embedded in either 0, 1.0 and 1.2% agar and cultured in vitro. The aggregation ... -
Development Of A Simple, Portable Carbon Dioxide Incubator For Production Of Bovine Ivf Embryos
Suzuki, Tatsuyuki | Sumantri, Cece | Boediono, Arief (1993)We have developed a simple, economical and portable C02 incubator for production of bovine IVF embryos in the field. The incubator consists of a metallic chamber (29L x 26W x 21 H em); the bottom plate of this chamber is ... -
Effect of gas atmosphere on the development of bovine embryos using a simple portable incubator
Khan, Nayeem Habeeb, A. | Kikkawa, Y | Sumantri, Cece | Saba, S | Murakami, MS | Boediono, Arief | Suzuki, Tatsuyuki (1993)The objective of this study was to examine the effect of gas atmosphere on the development of IVMFC of bovine embryos in a simple portable carbon dioxide incubator under negative and positve pressure of air. Incubation ... -
The effect of sperm-oocyte incubation time on in vitro embryo development using sperm from a tetraparental chimeric bull
Sumantri, Cece | Boediono, Arief | Ooe, Masato | Murakami, M. | Saha, S. | Suzuki, Tatsuyuki (1997)The present study was designed as 5 X 4 factorial to investigate the effects of using sperm from 5 bulls, and varied sperm-oocyte incubation times (5, 10, 15 and 20 h) on the fertilization, cleavage rates and blastocyst ... -
Effect Of The Presence Of A Cl In The Ovary On Oocyte Number, Cleavage Rate And Blastocyst Production In Vitro In Cattle
Boediono, Arief | Rajamahendran, Raja | Saha, S. | Sumantri, Cece | Suzuki, Tatsuyuki (1993)High progesterone concentration has been shown to cause regression of the dominant follicle and follicle wave turnover in cattle (Taylor and Rajamahendran, can J Anim Sci 74:281,1994). Pregnant cows also produced a higher ... -
Fertility of sperm from a tetraparental Chimeric bull
Sumantri, Cece | Boediono, Arief | Ooe, Masato | Saha, S. | Suzuki, Tatsuyuki (1996)The purpose of the present study was to examine the ability of a tetraparental Chimera in producing IVF embryos. Cumulus oocytes complexes (COCs) were matured in vitro for 22 h. Frozen-thawed sperm of a Chimera (CH), as ... -
Fertilization and Development of Frozen–Thawed Germinal Vesicle Bovine Oocytes by a One-Step Dilution Method in Vitro
Suzuki, T. | Boediono, Arief | Takagi, M. | Saha, S. | Sumantri, Cece (1996)The objective of this study was to evaluate in vitro fertilization and cleavage rates of frozen–thawed bovine oocytes at the germinal vesicle (GV) stage. In mouse oocytes, spindle microtubule reorganization after GV breakdown ... -
The influence of polyvinylpyrrolidone on freezing of bovine IVF blastocysts following biopsy
Suzuki, Tatsuyuki | Saha, S. | Sumantri, Cece | Takagi, M. | Boediono, Arief (1995)A study was conducted to develop a better freezing protocol for in vitro developed biopsled bovine blastocysts. Biopsied blastocysts were exposed to 1.8 M ethylene glycol (EG) + 0.05 M trehalose (T) and different their in ... -
Normal Calves Obtained after Direct Transfer of Vitrified Bovine Embryos Using Ethylene Glycol, Trehalose, and Polyvinylpyrrolidone
Saha, S. | M. Takagi | Boediono, Arief | Sumantri, Cece | Suzuki, Tatsuyuki (1996)In the present study, IVF bovine embryos were vitrified using as the cryoprotectants, ethylene glycol plus trehalose plus the polymer, polyvinylpyrrolidone (PVP). In Experiment I, toxicity of the vitrification solution ... -
The Role Of Pre-Equilibration For Vitrification Of In Vitro Produced (Ivp) Bovine Embryos
Saha, S. | Ooe, Masato | Boediono, Arief | Sumantri, Cece | M. Murakami | Suzuki, Tatsuyuki (1993)The role of equilibration for successful vitrification was investigated by one-step or three-step addition ard one-step removal of the cryoprotective agents (CPAs) in vitrification solution. The blastocysts ard expandedblastocysts ... -
Vitrification of bovine in vitro matured and pronuclear oocites with different vitrification solutions
Saha, S. | Boediono, Arief | Sumantri, Cece | M. Murakami | Y. Kikkawa | Suzuki, Tatsuyuki (1993)Vitrification of in vitro matured (IVM) and pronuclear (PN) oocytes was performed using three types of vitrification solutions (VS). IVM oocytes were vitrified with precooled (4°C) VS containing 40% ethylene glycol + 0.3M ...