Browsing Anatomy, Phisiology and Pharmacology by Author "Saha, S."
Now showing items 1-15 of 15
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The effect of sperm-oocyte incubation time on in vitro embryo development using sperm from a tetraparental chimeric bull
Sumantri, Cece | Boediono, Arief | Ooe, Masato | Murakami, M. | Saha, S. | Suzuki, Tatsuyuki (1997)The present study was designed as 5 X 4 factorial to investigate the effects of using sperm from 5 bulls, and varied sperm-oocyte incubation times (5, 10, 15 and 20 h) on the fertilization, cleavage rates and blastocyst ... -
Effect Of The Presence Of A Cl In The Ovary On Oocyte Number, Cleavage Rate And Blastocyst Production In Vitro In Cattle
Boediono, Arief | Rajamahendran, Raja | Saha, S. | Sumantri, Cece | Suzuki, Tatsuyuki (1993)High progesterone concentration has been shown to cause regression of the dominant follicle and follicle wave turnover in cattle (Taylor and Rajamahendran, can J Anim Sci 74:281,1994). Pregnant cows also produced a higher ... -
Fertility of sperm from a tetraparental Chimeric bull
Sumantri, Cece | Boediono, Arief | Ooe, Masato | Saha, S. | Suzuki, Tatsuyuki (1996)The purpose of the present study was to examine the ability of a tetraparental Chimera in producing IVF embryos. Cumulus oocytes complexes (COCs) were matured in vitro for 22 h. Frozen-thawed sperm of a Chimera (CH), as ... -
Fertilization and Development of Frozen–Thawed Germinal Vesicle Bovine Oocytes by a One-Step Dilution Method in Vitro
Suzuki, T. | Boediono, Arief | Takagi, M. | Saha, S. | Sumantri, Cece (1996)The objective of this study was to evaluate in vitro fertilization and cleavage rates of frozen–thawed bovine oocytes at the germinal vesicle (GV) stage. In mouse oocytes, spindle microtubule reorganization after GV breakdown ... -
Influence of day-0 and day-7 superovulated cow serum during development of bovine oocytes in vitro
Boediono, Arief | Takagi, M. | Saha, S. | Suzuki, Tatsuyuki (1994)Oocytes were matured in medium supplemented with 5% serum collected from superovulated cows at oestrus (Day-0 SCS) or at the time of embryo collection (Day-7 SCS), or in medium supplemented with fetal calf serum (FCS). ... -
The influence of polyvinylpyrrolidone on freezing of bovine IVF blastocysts following biopsy
Suzuki, Tatsuyuki | Saha, S. | Sumantri, Cece | Takagi, M. | Boediono, Arief (1995)A study was conducted to develop a better freezing protocol for in vitro developed biopsled bovine blastocysts. Biopsied blastocysts were exposed to 1.8 M ethylene glycol (EG) + 0.05 M trehalose (T) and different their in ... -
The Influence Of Sperm-Oocyte Incubation Time And Breed Of Bull On In Vitro Embryo Development In Catile
Sumantri, C. | Ooe, M. | Saha, S. | Boediono, Arief (1993)It has been reported that the length of sperm-oocyte-incubation time using Holstein sperm did not affect to the embryo development (Rehman, et al. Theriogenology 41:1447- 1452, 1994). Therefore, the present study was ... -
Normal Calves Obtained after Direct Transfer of Vitrified Bovine Embryos Using Ethylene Glycol, Trehalose, and Polyvinylpyrrolidone
Saha, S. | M. Takagi | Boediono, Arief | Sumantri, Cece | Suzuki, Tatsuyuki (1996)In the present study, IVF bovine embryos were vitrified using as the cryoprotectants, ethylene glycol plus trehalose plus the polymer, polyvinylpyrrolidone (PVP). In Experiment I, toxicity of the vitrification solution ... -
Pregnancy Rate And Survival In Culture Of In Vitro Fertilized Bovine Embryos Frozen In Various Cryoprotectants And Thawed Using A One-Step System
Suzuki, Tatsuyuki | M. Takagi | M. Yamamoto | Boediono, Arief | Saha, S. | H. Sakakibara | Ooe, Masato (1993)Bovine oocytes surrounded with compact cumulus cells were cultured for 20 to 22 hours (38.5°C, 5% C02) in modified TCM-199 medium supplemented with 5% superovulated cow serum (SCS) and inseminated by in vitro capacitated ... -
Production of chimeric calves by aggregation of in vitro-fertilized bovine embryos without zonae pellucidae
Boediono, Arief | M. Ooe | M. Yamamoto | M. Takagi | Saha, S. | Suzuki, Tatsuyuki (1993)Bovine embryos produced by in vitro maturation (IVM). fertilization (IVF) and culture (IVC) were used to produce aggregation chimeras. An aggregated chimera was produced by combining bovine IVF embryos (Holstein x Japanese ... -
The Role Of Pre-Equilibration For Vitrification Of In Vitro Produced (Ivp) Bovine Embryos
Saha, S. | Ooe, Masato | Boediono, Arief | Sumantri, Cece | M. Murakami | Suzuki, Tatsuyuki (1993)The role of equilibration for successful vitrification was investigated by one-step or three-step addition ard one-step removal of the cryoprotective agents (CPAs) in vitrification solution. The blastocysts ard expandedblastocysts ... -
Survival rate of frozan-thawed bovine IVF embryos in relation to exposure time using various cryoprotectans
M. Takagi | Boediono, Arief | Saha, S. | Suzuki, Tatsuyuki (1993)The objective of this study was to examine the relationship between cryoprotectant and equilibration t1me on development of IVF derived embryos in culture after thawing. Good to excellent quality day 1 to 8 blastocysts ... -
Viability Of Bovine Blastocysts Obtained After 7, 8 Or 9 Days Of Culture Following Vitrification And One-Step Rehydration
Saha, S. | Rajamahendran, R. | Boediono, Arief | Cece, Sumantri | Suzuki, T. (1993)This study examined the morphological appearance, hatching rates and live:dead cell ratios following vitrification of in vitro produced (IVP) bovine blastocysts. Expanded blastocysts obtained after 7, 8 or 9 d of culture ... -
Viability Of Frozen-Thawed Bovine Ivm/Ivf Embryos In Relation To Aging Using Various Cryoprotectants
Boediono, Arief | T. Otoi | M. Yamamoto | Saha, S. | Suzuki, Tatsuyuki (1994)Bovine IVF embryos developed on Days 7, 8 and 9 were equilibrated with 1. 6 M propylene glycol (PG) , 1. 8 M ethylene glycol (EG) , 1. 1 M diethylene glycol (DEG) or 1 . 3 M ethylene glycol monomethyl ether (EME) for 10 ... -
Vitrification of bovine in vitro matured and pronuclear oocites with different vitrification solutions
Saha, S. | Boediono, Arief | Sumantri, Cece | M. Murakami | Y. Kikkawa | Suzuki, Tatsuyuki (1993)Vitrification of in vitro matured (IVM) and pronuclear (PN) oocytes was performed using three types of vitrification solutions (VS). IVM oocytes were vitrified with precooled (4°C) VS containing 40% ethylene glycol + 0.3M ...