Browsing Anatomy, Phisiology and Pharmacology by Author "Ooe, Masato"
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The effect of sperm-oocyte incubation time on in vitro embryo development using sperm from a tetraparental chimeric bull
Sumantri, Cece | Boediono, Arief | Ooe, Masato | Murakami, M. | Saha, S. | Suzuki, Tatsuyuki (1997)The present study was designed as 5 X 4 factorial to investigate the effects of using sperm from 5 bulls, and varied sperm-oocyte incubation times (5, 10, 15 and 20 h) on the fertilization, cleavage rates and blastocyst ... -
Fertility of sperm from a tetraparental Chimeric bull
Sumantri, Cece | Boediono, Arief | Ooe, Masato | Saha, S. | Suzuki, Tatsuyuki (1996)The purpose of the present study was to examine the ability of a tetraparental Chimera in producing IVF embryos. Cumulus oocytes complexes (COCs) were matured in vitro for 22 h. Frozen-thawed sperm of a Chimera (CH), as ... -
Pregnancy rate and survival in culture of in vitro fertilized bovine embryos frozen in various cryoprotectants and thawed using a one-step system
Suzuki, Tatsuyuki | M. Takagi | M. Yamamoto | Saha. S. | Boediono, Arief | Ooe, Masato (1993)Bovine oocytes surrounded with compact cumulus cells were cultured for 20-22 h (38.5°C, 5 % C02) in modified TCM -199 supplemented with 5 % superovulated cow serum (SCS) and inseminated by in vitro capacitated sperm. Day ... -
Pregnancy Rate And Survival In Culture Of In Vitro Fertilized Bovine Embryos Frozen In Various Cryoprotectants And Thawed Using A One-Step System
Suzuki, Tatsuyuki | M. Takagi | M. Yamamoto | Boediono, Arief | Saha, S. | H. Sakakibara | Ooe, Masato (1993)Bovine oocytes surrounded with compact cumulus cells were cultured for 20 to 22 hours (38.5°C, 5% C02) in modified TCM-199 medium supplemented with 5% superovulated cow serum (SCS) and inseminated by in vitro capacitated ... -
The Role Of Pre-Equilibration For Vitrification Of In Vitro Produced (Ivp) Bovine Embryos
Saha, S. | Ooe, Masato | Boediono, Arief | Sumantri, Cece | M. Murakami | Suzuki, Tatsuyuki (1993)The role of equilibration for successful vitrification was investigated by one-step or three-step addition ard one-step removal of the cryoprotective agents (CPAs) in vitrification solution. The blastocysts ard expandedblastocysts ...