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isolation of oligosaccharides from local honey and analysis of its prebiotic activity

dc.contributor.authorKarimah, Umul
dc.contributor.authorAnggowo, Yogi Nur
dc.contributor.authorFalah, Syamsul
dc.contributor.authorSuryani
dc.date.accessioned2013-05-02T04:16:59Z
dc.date.available2013-05-02T04:16:59Z
dc.date.issued2013-05-02T04:16:59Z
dc.identifier.issn1978-1059
dc.identifier.urihttp://repository.ipb.ac.id/handle/123456789/63140
dc.descriptionJurnal Gizi dan Pangan, Nopember 2011, 6(3): 217-224en
dc.description.abstractThe objective of this study was to isolate oligosaccharides from local honey of Pulau Sumbawa and province of Kalimantan Timur and to analyze its prebiotic activity in vitro. Oligosaccharides were isolated with activated charcoal adsorption and ethanol treatment. Effectiveness of this method was evaluated by Thin Layer Chromatography and colorimetry. The isolates were then being a sample to prebiotic examination. Honey from Sumbawa forest has higher oligosaccharides content than honey from Kalimantan . Qualitative and quantitative assays showed that oligosaccharide isolation method was effective to concentrate the oligosaccharide with high degree aT polymerization but ineffective for mono-and disaccharides removal. The highest percentages of hydrolysis of oligosaccharide from Sumbawa and Kalimantan by mimic gastric juice were 1.78%and 0.59 %. Whereas, the hydrolysis was higher with amylase, 11.87% for Sumbawa and 21.03%for Kalimantan honey oligosaccharides. Prebiotic activity wQS 0.32, and 0_09 for honey oligosaccharide from Sumbawa and Kalimantan respectively. Honey oligosaccharide from Sumbawa presented higher prebiotic activity than inul in as prebiotic reference (0.11). GeMS chromatogram showed L. acidophilus cultured in media added with Sumbawa honey oligosaccharide produced lactic acid as the highest metabolite (48.01%).en
dc.description.sponsorshipPerhimpunan Peminat Gizi dan Pangan Indonesia dan Dept. Gizi Masyarakat, FEMA IPBen
dc.relation.ispartofseriesNopember 2011;Vol. 6, No. 3
dc.subjectoligosaccharideen
dc.subjectlocal honeyen
dc.subjectprebiotic activityen
dc.titleIsolasi oligosakarida madu lokal dan analisis aktivitas prebiotiknyaen
dc.titleisolation of oligosaccharides from local honey and analysis of its prebiotic activity
dc.typeOtheren


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