Induksi Mutasi Melalui Penggandaan Kromosom Nilam Varietas Sidikalang (Pogostemon cablin Benth.) dengan Kolkisin Secara In Vitro

Abstract

The research aimed to study the in vitro genetic mutation induction through chromosome doubling of patchouli (Pogostemon cablin Benth) using colchicine. This research was conducted from February 2011 to December 2011 at Biotechnology and Micro Technique Laboratory, Department of Agronomy and Horticulture, IPB, Bogor. The research was used factorial design which arranged with Completely Randomized Design. The research was consist of 2 factors, concentrations of colchicine (0, 0.02, 0.04 and 0.06%) and the long immersion with colchicine (24, 48, and 72 hours). The experiment showed that consentrations of colchicine (0.01, 0.02, 0.03, 0.04, and 0.05%) were significantly affected toincrease number of shoots, leaves, chloroplast, stomatas and size of stomata. Concentration of 0.04% colchicine with 24 immer was produced the highest number of shoots and leave. Concentration of 0.02% colchicine was produced the highest number of chloroplasts and the lowest density of stomata. Concentration of 0.06% of colchicine and 48 hours immersion was produced the biggest size of stomata.