Lingkage Analysis of RAPD (Random amplipied polymorphic DNA) Marker with fusaruim wilt resistance of watermelon (Citrullus lanatus (Tumberg) Maksum and Nakai)

Abstract

Random Amplified Polymorohic DNA (RAPD) analysis was aplied to analyze watermelon (Citrullus lanatus (Tunberg) Maksun and Nakai) using two pool extreem fusarium wilt resisten (Charleston-Gray, Long Dragon AG-13, New Long ET-03, and Sugar Baby-2 and pool exteem susceptible (Dragon Giant 145, Kaisar, dan Lokal kupang). 3 primer used for amplification contains P01, 109, and G10. The research shown that P01 could be linked and produced a different fragment polymorphic DNA between resisten and non-resisten population ao watermelon in 700bp. P01 was reported previosly as linked (1,6 cM) to race 1 fusarium wilt resistance in watermelon. The result by RAPD analysis showed that genotype of Charleston-Gray, long Dragon AG-14, New Long ET-03, and sugar Baby-2 susceptible fusarium wilt resistance gene, and genotype Dragon Giant 145, Kaisar, and Lokal kupang were resistance to fusarium wilt.