Identifikasi Golongan Darah Anjing Kampung dengan Antibodi Monoklonal

Abstract

A study was carried out to identify the blood groups of dogs and in determine their distribution among dog population as well as their possible relationship with hair color, sexes and location. Fifty dogs were used in this study and they were originated from Jakarta, Bekasi and Bogor, Indonesia. The blood groups of dogs were determined by monoclonal antibodies and a positive reaction was characterized by the presence of agglutination as a result of autigen-antibody reaction. Three blood groups, 1.1 B (44.0%), 1.2B (42.9%) and 1(-)B (14.0%) were detected among the 50 dogs examined. No significant relationship between certain the blood groups and the color, sexes and their origin were detected in this study.

Penelitian ini dilakukan untuk mengidentifikasi tipe dan sebaran golongan darah anjing kampung (Ganis familiares) di beberapa lokasi di Jakarta, Bekasi dan Bogor serta keterkaitannya dengan warna rambut, jenis kelamin dan lokasi anjing. Golongan darah anjing diidentifikasi dengan antibodi monoklonal produksi Shigeta. Sebanyak 50 ekor anjing kampung digunakan sebagai sampel dalam penelitian ini. Penentuan golongan darah ditentukan berdasarkan adanya reaksi aglutinasi antara antigen dan antibody. Hasil pemeriksaan di dapatkan tiga golongan darah yaitu 1.1B (44.0%), 1.2B (42.0%) dan 1(-)B (14.0%) pada 50 ekor anjing. Dalam penelitian ini tidak ditemukan kaitan antara warna rambut, jenis kelamin dan lokasi terhadap kecenderungan munculnya golongan darah tertentu.