Microsatellite Markers for Classifying and Analysing Genetic Relationship between Banana Cultivars in Indonesia
Abstract
In this study, microsatcllite markers were used to identify Musa genomic groups and to investigate genetic relationships among banana accessions from \Brious areas in lndoncsia. One hundred sixteen b.muna accessions were analysed using MaCrRl08 and Ma-3-90 primer pairs for identifying genomic groups. Six additional SSR primer pairs were used for genetic rehltionship analysis. The results showed that 73 accessions should be classified in the AA/AAA and AAA genomic groups, two accessions in the SS genomic group, 21 accessions in the AAB genomic group and 20 accessions in the ASB genomic group. Ninety-nine out of the 116 accessions were unique genotypes while the rest were synonyms. The dendrogram generated by UPGMA analysis separated the 116 banana accessions into 1"\'1-'0 main clusters with a similarity of 0.13. All banana accessions belonging to the SB, ABB and AAB genomic groul)s clustered in the first main cluster, together with the majority of the accessions conhlining the pure A genome. The second main cluster ~as formed of 1 t accessions of AAJAAA and AAA genome. Within the first main cluster, the accessions containing the B genome were clustered according to their genomic group, e.xcept four AAB accessions clustering with accessions containing the A genome alone. The ABB genomic group ~lppeared closer to the SS than to the AAB genomic group. The AA and AAA banana accessions could not be significantly distinguished, although the majority of accessions tended to be clustered according to their ploidy level.
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