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dc.contributor.authorAstuti, Rika Indri
dc.contributor.authorWahyudi, Aris Tri
dc.contributor.authorMubarik, Nisa Rachmania
dc.date.accessioned2012-05-16T04:12:49Z
dc.date.available2012-05-16T04:12:49Z
dc.date.issued2006
dc.identifier.issn0853-358X
dc.identifier.urihttp://repository.ipb.ac.id/handle/123456789/54546
dc.description.abstractAn acid-aluminium sensitive mutant of Bradyrhizobium japonicum 38. designated as AAS38, was generated by mini-Tn5 transposon mutagenesis. The experiment was carried out to identify acid-aluminium tolerance gene (AAT) in B. japonicum. Transposon delivery was carried out through conjugation between Escherichia coli S17-I (λ pir) carrying pUTmini-Tn5Kml and acid-Al tolerant B. japonicum with different of mating time. Frequency of transconjugation was in the range of 10-7 -10-6. A mutant AAS38 was not able to grow on the Ayanaba medium (pH 4.5) containing 50 µM aluminium. However, this mutant formed root nodule of soybean and Siratro plants indicating the gene involved in acid-Al tolerance was not related with nodulation. A 0.8 kb of the genomic DNA fragment flanking the transposon involved in acid-aluminium tolerance was successfully isolated by inverse polymerase chain reaction (Inverse PCR) from AAS38 genome. This fragment was subsequently cloned into pGEM-T Easy (~3 kb) to yield a recombinant plasmid, designated as pGEMT-38 (~3.8 kb), and sequenced. DNA sequence analysis revealed that the genomic DNA fragment had high homology to inner membrane protein from Salmonella typhimurium (80% identity and 86% similarity, E-value= 8xe-62 predicted function as efflux transporter.en
dc.publisherIPB (Bogor Agricultural University)
dc.subjectBradyrhizobium japonicum, acid-aluminium tolerance, transposom mutagenesis, cloningen
dc.titleCloning of Genomic DNA Fragment Involve in Acid-Aluminium Tolerance in Bradyrhizobium japonicum 38 Through Transposom Mutagenesisen
dc.title.alternativeJurnal Mikrobiologi Indonesiaen


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