Identification of coxiella burnetii in ruminants by nested-polymerase chain reaction in Indonesia

Abstract

Nested-polymerase chain reaction (Ned-PCR) was used to identtfy Coxiella bumetii, a causal agent of Q fever, a zoonotic disease during 2004 to 2006 in Indonesia. A total of 410 sample organs from sfaughtemouses such as liver and lung of cattle (245 samples), sheep (105 samples) and goat (60 samples) from Bogor area and Bali province were investigated. The result showed that 5.12% of ruminant samples tested were positive C.bumeiii DNA that consist of 15 cattle samples (3.66%) and 6 sheep samples (1.46%), and no positive result from goat Interestingly, 3 of 95 positive cattle samples were Bali's cattle. The evidence showed that C.bvmetii maybe widespread in Indonesia. This is the first report of identification of C.bumetii from livestock in Indonesia.