Faculty of Veterinary: Recent submissions
Now showing items 161-180 of 340
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Effect of gas atmosphere on the development of bovine embryos using a simple portable incubator
(1993)The objective of this study was to examine the effect of gas atmosphere on the development of IVMFC of bovine embryos in a simple portable carbon dioxide incubator under negative and positve pressure of air. Incubation ... -
Fertility of sperm from a tetraparental Chimeric bull
(1996)The purpose of the present study was to examine the ability of a tetraparental Chimera in producing IVF embryos. Cumulus oocytes complexes (COCs) were matured in vitro for 22 h. Frozen-thawed sperm of a Chimera (CH), as ... -
Chimera production in bovine and its sperm capacity
(1998)In vitro produced bovine embryos were used to produce chimeras by combining the 8- cell stage embryos. Aggregated embryos were embedded in either 0, 1.0 and 1.2% agar and cultured in vitro. The aggregation ... -
Perkembangan in vitro dan in vivo embrio mencit tanpa zona pelusida
(1999)The aim of this study was to examine the in vitro and in vivo development of zona pellucida•free mouse embryos transferred to the recipient. Embryos were flushed on 4 days of gestation (vaginal plug=1 day of gestation) ... -
Produksi embrio kambing dengan teknologi maturasi, fertilisasi dan kultur in vitro
(1999)Penelitian ini bertujuan meneliti perkembangan oosit kambing setelah maturasi, fertilisasi dan kultur in vitro dalam upaya produksi embrio kambing. Oosit dikoleksi melalui aspirasi ovarium yang memiliki ... -
The influence of polyvinylpyrrolidone on freezing of bovine IVF blastocysts following biopsy
(1995)A study was conducted to develop a better freezing protocol for in vitro developed biopsled bovine blastocysts. Biopsied blastocysts were exposed to 1.8 M ethylene glycol (EG) + 0.05 M trehalose (T) and different their in ... -
Survival rate of frozan-thawed bovine IVF embryos in relation to exposure time using various cryoprotectans
(1993)The objective of this study was to examine the relationship between cryoprotectant and equilibration t1me on development of IVF derived embryos in culture after thawing. Good to excellent quality day 1 to 8 blastocysts ... -
Fertilization and Development of Frozen–Thawed Germinal Vesicle Bovine Oocytes by a One-Step Dilution Method in Vitro
(1996)The objective of this study was to evaluate in vitro fertilization and cleavage rates of frozen–thawed bovine oocytes at the germinal vesicle (GV) stage. In mouse oocytes, spindle microtubule reorganization after GV breakdown ... -
Normal Calves Obtained after Direct Transfer of Vitrified Bovine Embryos Using Ethylene Glycol, Trehalose, and Polyvinylpyrrolidone
(1996)In the present study, IVF bovine embryos were vitrified using as the cryoprotectants, ethylene glycol plus trehalose plus the polymer, polyvinylpyrrolidone (PVP). In Experiment I, toxicity of the vitrification solution ... -
Production Of Chimeric Calves Derived From Parthenogenetic And Fertilized Bovine Embryos
(1993)The present study was aimed to examine whether parthenogenetic cells could contribute to the development of bovine embryos to term. Chimeric blastocysts were produced by aggregation of parthenogenetic and in vitro fertilized ... -
The Role Of Pre-Equilibration For Vitrification Of In Vitro Produced (Ivp) Bovine Embryos
(1993)The role of equilibration for successful vitrification was investigated by one-step or three-step addition ard one-step removal of the cryoprotective agents (CPAs) in vitrification solution. The blastocysts ard expandedblastocysts ... -
Calves Obtained After Transfer Of Frozen-Thawed Bovine Immature Oocytes Cryopreserved In Various cryoprotectants
(1993)The objective of this study was to evaluate the fertilization and cleavage rates in vitro of frozen-thawed bovine oocytes at the germinal vesicle (GV) stage. Various permeating cryoprotective agents [1.8M ethylene glycol ... -
The Influence Of Sperm-Oocyte Incubation Time And Breed Of Bull On In Vitro Embryo Development In Catile
(1993)It has been reported that the length of sperm-oocyte-incubation time using Holstein sperm did not affect to the embryo development (Rehman, et al. Theriogenology 41:1447- 1452, 1994). Therefore, the present study was ... -
Aggregation of parthenogenetic and fertilized embryos for producing chimera calves
(1993)Chimeric blastocysts were produced by aggregation of parthenogenetic and fertilized demi-embryos from Japanese Brown breed and Holstein breed cattle, respectively. The fertilized embryos were obtained by in vitro maturation, ... -
Vitrification of bovine in vitro matured and pronuclear oocites with different vitrification solutions
(1993)Vitrification of in vitro matured (IVM) and pronuclear (PN) oocytes was performed using three types of vitrification solutions (VS). IVM oocytes were vitrified with precooled (4°C) VS containing 40% ethylene glycol + 0.3M ... -
Development Of A Simple, Portable Carbon Dioxide Incubator For Production Of Bovine Ivf Embryos
(1993)We have developed a simple, economical and portable C02 incubator for production of bovine IVF embryos in the field. The incubator consists of a metallic chamber (29L x 26W x 21 H em); the bottom plate of this chamber is ... -
Viability Of Bovine Blastocysts Obtained After 7, 8 Or 9 Days Of Culture Following Vitrification And One-Step Rehydration
(1993)This study examined the morphological appearance, hatching rates and live:dead cell ratios following vitrification of in vitro produced (IVP) bovine blastocysts. Expanded blastocysts obtained after 7, 8 or 9 d of culture ... -
Effect Of The Presence Of A Cl In The Ovary On Oocyte Number, Cleavage Rate And Blastocyst Production In Vitro In Cattle
(1993)High progesterone concentration has been shown to cause regression of the dominant follicle and follicle wave turnover in cattle (Taylor and Rajamahendran, can J Anim Sci 74:281,1994). Pregnant cows also produced a higher ... -
Viability Of Frozen-Thawed Bovine Ivm/Ivf Embryos In Relation To Aging Using Various Cryoprotectants
(1994)Bovine IVF embryos developed on Days 7, 8 and 9 were equilibrated with 1. 6 M propylene glycol (PG) , 1. 8 M ethylene glycol (EG) , 1. 1 M diethylene glycol (DEG) or 1 . 3 M ethylene glycol monomethyl ether (EME) for 10 ... -
Production of chimeric calves by aggregation of in vitro-fertilized bovine embryos without zonae pellucidae
(1993)Bovine embryos produced by in vitro maturation (IVM). fertilization (IVF) and culture (IVC) were used to produce aggregation chimeras. An aggregated chimera was produced by combining bovine IVF embryos (Holstein x Japanese ...
