Search
Now showing items 1-10 of 16
Survival rate of frozan-thawed bovine IVF embryos in relation to exposure time using various cryoprotectans
(IPB (Bogor Agricultural University), 1993)
The objective of this study was to examine the relationship between cryoprotectant and equilibration t1me on development of IVF derived embryos in culture after thawing. Good to excellent quality day 1 to 8 blastocysts ...
The Role Of Pre-Equilibration For Vitrification Of In Vitro Produced (Ivp) Bovine Embryos
(IPB (Bogor Agricultural University), 1993)
The role of equilibration for successful vitrification was investigated by one-step or three-step addition ard one-step removal of the cryoprotective agents (CPAs) in vitrification solution. The blastocysts ard expandedblastocysts ...
Viability Of Frozen-Thawed Bovine Ivm/Ivf Embryos In Relation To Aging Using Various Cryoprotectants
(IPB (Bogor Agricultural University), 1994)
Bovine IVF embryos developed on Days 7, 8 and 9 were equilibrated with 1. 6 M propylene glycol (PG) , 1. 8 M ethylene glycol (EG) , 1. 1 M diethylene glycol (DEG) or 1 . 3 M ethylene glycol monomethyl ether (EME) for 10 ...
Effect Of The Presence Of A Cl In The Ovary On Oocyte Number, Cleavage Rate And Blastocyst Production In Vitro In Cattle
(IPB (Bogor Agricultural University), 1993)
High progesterone concentration has been shown to cause regression of the dominant follicle and follicle wave turnover in cattle (Taylor and Rajamahendran, can J Anim Sci 74:281,1994). Pregnant cows also produced a higher ...
Influence of day-0 and day-7 superovulated cow serum during development of bovine oocytes in vitro
(Bogor Agriculture University, 1994)
Oocytes were matured in medium supplemented with 5% serum collected from superovulated cows at oestrus (Day-0 SCS) or at the time of embryo collection (Day-7 SCS), or in medium supplemented with fetal calf serum (FCS). ...
The effect of sperm-oocyte incubation time on in vitro embryo development using sperm from a tetraparental chimeric bull
(IPB (Bogor Agricultural University), 1997)
The present study was designed as 5 X 4 factorial to investigate the effects of using sperm from 5 bulls, and varied sperm-oocyte incubation times (5, 10, 15 and 20 h) on the fertilization, cleavage rates and blastocyst ...
Vitrification of bovine in vitro matured and pronuclear oocites with different vitrification solutions
(IPB (Bogor Agricultural University), 1993)
Vitrification of in vitro matured (IVM) and pronuclear (PN) oocytes was performed using three types of vitrification solutions (VS). IVM oocytes were vitrified with precooled (4°C) VS containing 40% ethylene glycol + 0.3M ...
The Influence Of Sperm-Oocyte Incubation Time And Breed Of Bull On In Vitro Embryo Development In Catile
(IPB (Bogor Agricultural University), 1993)
It has been reported that the length of sperm-oocyte-incubation time using Holstein sperm did not affect to the embryo development (Rehman, et al. Theriogenology 41:1447- 1452, 1994). Therefore, the present study was ...
Fertilization and Development of Frozen–Thawed Germinal Vesicle Bovine Oocytes by a One-Step Dilution Method in Vitro
(IPB (Bogor Agricultural University), 1996)
The objective of this study was to evaluate in vitro fertilization and cleavage rates of frozen–thawed bovine oocytes at the germinal vesicle (GV) stage. In mouse oocytes, spindle microtubule reorganization after GV breakdown ...
Production of chimeric calves by aggregation of in vitro-fertilized bovine embryos without zonae pellucidae
(IPB (Bogor Agricultural University), 1993)
Bovine embryos produced by in vitro maturation (IVM). fertilization (IVF) and culture (IVC) were used to produce aggregation chimeras. An aggregated chimera was produced by combining bovine IVF embryos (Holstein x Japanese ...