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      Penapisan Inhibitor Protease Yang Dihasilkan Oleh Sponge Asal Kepulauan Seribu

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      Date
      2004
      Author
      Nurhayati, Tati
      Suptijah, Pipih
      Suhartono, Maggy T.
      Febrian, Irman
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      Abstract
      Several medicines showing protease inhibitor mechanism are widely available in the markets. Among marine organisms, sponge is the big producer of bioactive compound, include protease inhibitor. The purpose of this research was to screen of protease inhibitor produced by sponge. Screening was be conducted using agar diffusion methods on ten of sponge which be extracted with methanol and distilled water. As test bacteria were used three species of pathogenic bacteria that is Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus. The result of research showing that sponge which be extracted using methanol have low protease inhibitory activity (< 30%), while that which be extracted using distilled water have high protease inhibitory, that is extract of Jaspis stellifera and extract of Plakortis nigra (> 50%). The extract of Jaspis stellifera was inhibited of protease from Escherichia.coli and Staphylococcus aureus by minimum inhibitory concentration (MIC) 0.08%, while that extract of Plakortis nigra was inhibited protease from S. aureus by MIC 0.12%. Ethylenediamine tetraacetic acid (EDTA) was inhibited S. aureus and 'E. coli by MIC 0,16%. Based on the data, can be concluded that both the extract of sponge were potential as protease inhibitor.
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      http://repository.ipb.ac.id/handle/123456789/53049
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      Indonesia DSpace Group 
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