Kemampuan Streptomyces spp. dalam Menghambat Pertumbuhan Mikrob Patogen Tular Tanah: Pengaruh Media dan Waktu Pertumbuhan (Capability of Streptomyces Spp in Inhibiting Soil Borne Microbial Pathogens:the Influence of Growth Media and Incubation Times)

Abstract

Streptomyces spp. has the potency to be used as an agent of biological control through their capability to produce various anti microbial compounds. The production of anti microbial compounds can be influenced by nutrition of culture media and incubation times. This research aimed to study the capability of Streptomyces spp. in inhibiting soil borne microbial pathogen influenced by growth media and incubation times. Two tested media i.e: ISP4 and modified molasses-soy bean meal media; and 5, 10, and 15 days of incubation times. Three local isolates of Streptomyces namely LSW05, LBR02 and PS4- 16 grew better and produced more anti microbial activity when they grown using modified molasses-soy bean meal media compared with that using ISP4. TTie highest anti microbial activity was obtained at 15 days incubation at room temperature. The three selected Streptomyces isolates was able to inhibit bacterial pathogen (Xanthomonas oryzae) and fungal pathogen (Fusarium oxysporum). Three local isolates of Streptomyces spp. produce antimicrobial compounds, and its production can be stimulated by giving suitable environmental growth conditions.