| dc.contributor.author | Artika, I Made | |
| dc.date.accessioned | 2011-03-28T02:52:15Z | |
| dc.date.available | 2011-03-28T02:52:15Z | |
| dc.date.issued | 2006 | |
| dc.identifier.issn | 0854-8587 | |
| dc.identifier.uri | http://repository.ipb.ac.id/handle/123456789/43281 | |
| dc.description.abstract | Subunit 8 of yeast mitochondrial ATP synthase is a polypeptide of 48 amino acids encoded by the mitochondrial ATP8 gene. A nuclear version of subunit 8 gene has been designed to encode FLAG tagged-subunit 8 fused with a mitochondrial signal peptide. The gene has been cloned into a yeast expression vector and then expressed in a yeast strain lacking endogenous subunit 8. Results showed that the gene was successfully expressed and the synthesized FLAG taggedsubunit 8 protein was imported into mitochondria. Following import, the FLAG tagged-subunit 8 protein assembled into functional mitochondrial ATP synthase complex. Furthermore, the subunit 8 protein could be detected using anti-FLAG tag monoclonal antibody. | en |
| dc.publisher | IPB (Bogor Agricultural University) | |
| dc.relation.ispartofseries | Vol 13;No 1 | |
| dc.title | Allotopic expression of a gene encoding FLAG Tagged-subunit 8 of yeast mitochondrial ATP synthase | en |
| dc.title.alternative | HAYATI Journal of Biosciences Vol. 13 No.1 Tahun 2006 | en |
