dc.contributor.author | Rahayu, Puji | |
dc.contributor.author | Setyahadi, Siswa | |
dc.contributor.author | Harmita | |
dc.date.accessioned | 2011-03-23T04:08:30Z | |
dc.date.available | 2011-03-23T04:08:30Z | |
dc.date.issued | 2008 | |
dc.identifier.issn | 1978-3477 | |
dc.identifier.uri | http://repository.ipb.ac.id/handle/123456789/42942 | |
dc.description.abstract | Xylanase (endoxylanase, EC 3.2.1.8) is a commercial enzyme that has been applied in the industrial production of fuel, food, textiles and paper. Xylanase was isolated from the culture supernatant of Bacillus sp. AQ-1 grown on Nakamura medium containing 0.5% powder bunch palm oil. The optimum pH and temperature of xylanase activity were pH 7.0 and 60 °C, respectively. The enzyme was purified by anion exchange chromatography using DEAE-Sepharose-Fast-Flow column and gel filtration chromatography using Sephacryl S-300 column. The results showed that purification of xylanase produced two forms of xylanase, which were identified as xylanase A and xylanase B. Xylanase A can be separated from xylanase B by ultrafiltration using a 30 kDa polyethersulfone membrane. The molecular weight of xylanase A and B were 15.7 and 57.7 kDa, respectively. | en |
dc.publisher | IPB (Bogor Agricultural University) | |
dc.relation.ispartofseries | Vol.3;No.1 | |
dc.title | Production and Purification of Xylanase From Indonesian Isolate Bacillus sp. AQ-1 Grown on Bunch Palm Oil | en |
dc.title.alternative | Microbiology Indonesia Vol.3 No.1 Tahun 2008 | en |