dc.contributor.author | Yuhana, Munti | |
dc.contributor.author | Hanselmann, Kurt | |
dc.date.accessioned | 2011-03-23T03:28:45Z | |
dc.date.available | 2011-03-23T03:28:45Z | |
dc.date.issued | 2008 | |
dc.identifier.issn | 1978-3477 | |
dc.identifier.uri | http://repository.ipb.ac.id/handle/123456789/42922 | |
dc.description.abstract | We collected the culturable heterotrophic bacteria from oligotrophic high mountain lake habitats and tested their capability to induce ice formation. Direct plating was carried out using low-nutrient medium at a temperature of between 3 and 4°C. As many as 84 isolates were recovered from glacial ice and natural biofilm growing on granite rocks surface. Six out of 84 isolates were capable of expressing the ice-nucleation phenotype. After autoclaving the cell suspension at 121°C for 15 min, isolate J78 was still able to retain the ability for ice formation. Heat-stable ice nuclei produced by ice-nucleating active bacteria have potential applications in biotechnology. Characterization of INA bacteria was performed employing live-dead Gram staining and molecular methods. Universal primers for Bacteria (S-D-Bact-0008-b-S-20 and S-D-Bact-1524-a-A-18) were used for PCR to amplify almost the full length of the 16S rRNA genes of selected INA isolates. Restriction fragment length polymorphism analysis resulted in 2 unique patterns, as represented by J43 and J83, respectively. Based on DNA sequencing of 16S rRNA gene, isolate J43 (GeneBank accession no. AJ864852) was closely related to Pseudomonas mephitica (99.2% sequence similarity) and Janthinobacterium lividum (99% similarity), whereas isolate J83 (GeneBank accession no. AJ864859) showed 100% sequence identity to Pseudomonas fluorescens. | en |
dc.publisher | IPB (Bogor Agricultural University) | |
dc.relation.ispartofseries | Vol.2;No.3 | |
dc.title | Free-Living Ice-Nucleating Active Bacteria from High Mountain Lake Habitats | en |
dc.title.alternative | Microbiology Indonesia Vol.2 No.3 Tahun 2008 | en |