View Item 
      •   IPB Repository
      • IPB e-Journal
      • Journal of Microbiology Indonesia
      • View Item
      •   IPB Repository
      • IPB e-Journal
      • Journal of Microbiology Indonesia
      • View Item
      JavaScript is disabled for your browser. Some features of this site may not work without it.

      Role of Bacteria in Tempe Bitter Taste Formation: Microbiological and Molecular Biological Analysis Based on 16S rRNA Gene

      Thumbnail
      View/Open
      e-Journal (48.62Kb)
      Date
      2008
      Author
      Barus, Tati
      Suwanto, Antonius
      Wahyudi, Aris Tri
      Wijaya, C. Hanny
      Metadata
      Show full item record
      Abstract
      Tempe is traditional Indonesian food. It has a variety of tastes, sometimes with a hint of bitterness, which may differ in intensity. The cause of bitterness in tempe has never been reported previously. In this study, the aim is to identify whether bacteria play a role in the formation of bitter tastes in tempe. Sensory tests were carried out in order to determine the scores of bitter-taste-intensity in tempe. The sensory test on EMP, WJB, CLR, DRG, and MLB tempe shows that EMP tempe has the highest score (2.3) and WJB has the lowest (1.3). It is revealed that the processing method has no impact on the formation of the bitter taste in tempe. Plating analysis, showed that EMP soaking water contained a higher number of Enterobacteria group bacteria, approximately 103-104 CFU ml-1 and spore-forming bacteria groups, 102 CFU ml-1, compared to WJB. Similarly, other bacteria groups in fresh EMP tempe was 102 CFU g-1 higher than those in fresh WJB tempe. Based on sequencing the16S rRNA gene, the dominant bacteria on PCA media in EMP tempe are Acetobacter indonesiensis, Klebsiella pneumoniae, Bacillus subtilis, and Flavobacterium sp. On the other hand those in WJB tempe were Klebsiella sp., Brevundimonas sp., Bacillus sp., Pseudomonas putida, and Acinetobacter sp. Bacillus, a group of proteolytic bacteria was found 105 CFU m-1 higher in the soaking water of EMP compared to WJB. Nevertheless, the types and numbers of fungi were not significantly different between tempe types. Accordingly, it is concluded that the difference in the number and the types of bacteria involved in the tempe production process leads to the difference in the bitter taste intensity in both EMP and WJB tempe.
      URI
      http://repository.ipb.ac.id/handle/123456789/42896
      Collections
      • Journal of Microbiology Indonesia [153]

      Copyright © Central Library of Bogor Agricultural University.
      Jl. Raya Darmaga Kampus IPB Darmaga Bogor 16680 West Java Indonesia
      All rights reserved. 2017
      Contact Us | Send Feedback
      Indonesia DSpace Group 
      IPB Scientific Repository
      UIN Syarif Hidayatullah Institutional Repository
      Universitas Jember Digital Repository
        

       

      Browse

      All of IPB RepositoryCollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

      My Account

      Login

      Application

      google store

      Copyright © Central Library of Bogor Agricultural University.
      Jl. Raya Darmaga Kampus IPB Darmaga Bogor 16680 West Java Indonesia
      All rights reserved. 2017
      Contact Us | Send Feedback
      Indonesia DSpace Group 
      IPB Scientific Repository
      UIN Syarif Hidayatullah Institutional Repository
      Universitas Jember Digital Repository