View Item 
      •   IPB Repository
      • IPB e-Journal
      • Journal of Microbiology Indonesia
      • View Item
      •   IPB Repository
      • IPB e-Journal
      • Journal of Microbiology Indonesia
      • View Item
      JavaScript is disabled for your browser. Some features of this site may not work without it.

      The Dynamics of Bacterial Communities During Traditional Nata de Coco Fermentation

      Thumbnail
      View/Open
      e-Journal (579.6Kb)
      Date
      2007
      Author
      Seumahu, Cecilia Anna
      Suwanto, Antonius
      Hadisusanto, Debora
      Suhartono, Maggy Thenawidjaja
      Metadata
      Show full item record
      Abstract
      One of the important problems in traditional Nata de Coco (Nata) fermentation is production inconsistency due to strain or genetic variability reflecting mixed microbial communities involved in this process. This research was aimed at examine the population dynamics of the bacterial community during the fermentation processes. Samples were collected daily for six days from fermentation media derived from “good” and “bad” Nata fermentation. We compared the levels of bacterial diversity through amplified 16S-rRNA (ARDRA). DNA was extracted directly from the fermentation media and 16S-rRNA gene was amplified employing Universal Bacterial Primers. The amplicons were cloned into pGEM-T Easy vector, and restriction enzymes HaeIII and RsaI were used to generate ARDRA profiles. ARDRA phylotypes of DNA extracted from the fermentation medium obtained from different Nata qualities were compared. Phylotype profiles demonstrated unique bacterial community profiles for different conditions of Nata quality, which could be developed as a parameter to monitor Nata quality during fermentation. In this research we found that the dynamics of the bacterial population involved in Nata fermentation were a crucial factor for determining traditional Nata quality.
      URI
      http://repository.ipb.ac.id/handle/123456789/42866
      Collections
      • Journal of Microbiology Indonesia [153]

      Copyright © 2020 Library of IPB University
      All rights reserved
      Contact Us | Send Feedback
      Indonesia DSpace Group 
      IPB University Scientific Repository
      UIN Syarif Hidayatullah Institutional Repository
      Universitas Jember Digital Repository
        

       

      Browse

      All of IPB RepositoryCollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

      My Account

      Login

      Application

      google store

      Copyright © 2020 Library of IPB University
      All rights reserved
      Contact Us | Send Feedback
      Indonesia DSpace Group 
      IPB University Scientific Repository
      UIN Syarif Hidayatullah Institutional Repository
      Universitas Jember Digital Repository