Study on the Cultivation Technique and the Effect of Surface Area of Growth Media to the Production of Cellulose by Indigenous Isolates

Abstract

RBP-52 and P15 isolates were Isolated from Indonesian fruits samples that contains glucose. RBP-52 Isolate was obtained from coconut water waste, collected from nata factory, and PDS Isolate was obtained from rotten coconut sample, collected from Pansng. They were identified as Acetobacter liquefaciens. RBP-52 and PD5 Isolate produced cellulose in static and shaking cultivation. Production of cellulose by RBP 52 and P15 were optimized in static cultivation. Cellulose was found to be produced at the liquid surface In static cultivation. The rate of cellulose production depended proportionally on the surface area or the culture medium. The maximum production rate of cellulose was 2.78 gIl per 7 days surface area 77.60 cm3 in the static cultivation.