Cultivation of Thermophilic Bacteria Isolate of a-Amylase Production
Yusuf, Gagan Maulana
Damardjati, Djoko Said
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Cultivation of thermophilic bacteria isolate TVII-6 for a.amylase production. Thermophilic bacteria TVIJ-6 was isolated and selected from the soil sample taken from Dieng vulcanic. The specific activity of ainylase produced by TVfl-6 was 1624.37 U/mg protein. Cassava starch was used as carbon source on enrichment culture and the optimum concentration was 1% with specific activity 2092.23 U/mg protein. Cultivation in a 2-I bioreactor at pH 7.0 and temperature of 50°C, combine with 250 rpm agitation and 1.0 vvm aeration produced the highest a.amylase. The maximum specific growth rate obtained was 0.147/hours. The relationship between product and substrate was linear Y=0.129 X + 0.024. Efficiency of the substrate to produce amylase Y, was 0.129 g proteinlg substrate. Relationship between the bacterial growth and substrate was Y = 03115 X + 0.013. Efficiency of the substrate for growing the bacteria Y,, was 0.315 g cell/g substrate. Based on the bacterial growth and amylase production, amylase could be considered growth associated, with kinetic parameter of product ratep was 0.37gfgfhr.