Fed-Batch Fermentation Processes to Produce dextranase fro of Streptococcus sp.B7
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The research aimed to develop fermentation technique on dextranase production from the bacterial isolate of Streptococcus sp. B7 using fed-batch Fermentation system. The production was done in two litre Fermentor with flow rate of medium addition at 19 mI/hr after 24 hours until 72 hours of incubation process. The variable conditions were pH of 7 and 8 and agitation speeds of 300 and 500 rpm. Maximum production was achieved at 500 rpm and p11 8 that produced enzyme activity of 920 U/mI. The best result of some kinetic parameters were as follows : production p = 920 U/mI, productivity Qv = 16.43 U/mllhr, dextranase specific activity 17.68 U/mg protein, yield of product over cell Yp/x = 696.47 U/g cell and specific productivity q, = 12.45 U/g celllhr.