| dc.description.abstract | Some researches indicated that oryzanol had anboxldant actlvity, however, the information about the oryzanol role ln prevention of low dens~ty Iipoprote~n (LDL) and human lymphocyte from ox~dahon under oxidative stress was still I~mited The objecbve of this study was to invesl~gate the antiox~dant actlvlty of oryzanol at concentratlons based on rice bran beverage model ln preventing LDL and lymphocyte from oxidation Human plasma were supplemented wlth the samples of nce bran 011 [RBO), unsaponifiable matter and oryzanol IR-64, oryzanol IR-64 3x and oryzanol standard at the concentratlons of 308 3, 22 2, 5 2, 10 4 and 10 4 pglml, respectively Afterward, the human LDL were collected by ultracentnfuge and d~lutedu nbl a concentrat~ono f 200 pg prote~n/mlw as reached Human LDL lsolates were then oxidlzed with CuS04 5 /iM for measuring antloxldant activlty of the samples The length of incubation, H202 concentration, penod of sample supplemented Into human lymphocyte culture were determined before the antiox~dant actlvity of RBO and its fractlon in lymphocyte was measured The samples used in the lymphocyte were RBO IR-64, unsapon~f~abmlea tter 1/3-64, and oryzanol standard at the concentratlons of 133 2 - 2,132 0 ~rglml,9 6 - 153 6 lig/ml, and 2 4 - 37 7,ug/rnl, consecut~vely The result showed that malonaldehyde concentration m human LDL decreased significantly (~s= 0 05), 15 - 41°0 and 39 - 56 % compared to the control The absorbance of 11v1ngly mphocyte cell ln culture was not influenced by the type and concentrat~ono f RE0 and its fracbon The addltlon of hydrogen peroxlde (Hz04 3 mM into culture s~gnlf~cantlloyw ered the absorbance as compared to culture w~thoutH 202 | en |
