Kualitas Semen Beku Kuda Pada Pengencer Susu Skim Dengan Konsentrasi Gliserol Yang Berbeda

Abstract

Cryoprotectant is one of determining factors for the success of stallion semen cryopreservation. For most mammalian sperm cryopreservation,glycerol has been widely used as the cryoprotectant. Glycerol in high concentration is toxic to the sperm but in low concentration it has protective effect on the semen. The objectives of this experiment were to find appropiate glycerol concentration by using skim milk extender in order to maintain motility and viability of spermatozoa of stallion frozen semen. Semen was collected from three sexually mature stallions by using artificial vagina. The semen characteristics and quality were examined both macro and microscopically. Prior to extension, semen was centrifugated at 3000 rpm for 15 minutes. The pellet was re-extended with skim milk extender with glycerol 5% (G5) 7.5% (G7,5), and 10% (G10). The extended semen as then packed in mini straw (0.3ml), equilibrated at 5 "C for 2 hours, frozen in the liquid N, vapor for15 minutes and then stored in liquid N, container until futher evaluation. The frozen semen, was thawed at 37 "C for 30 seconds. The percentages of sperm motility and viability were examihed. The result of this research showed that the sperm motility and viability in G5 was (23.8 %; 35.6 %) significantly hihger (P<0.05) as compare a with G7.5(18.1%; 28.6%) and G10. (11.8%); 23.1%). The recovery Rate in G5 (35.4%) was significant high er (Pi0.05) than G5, (26.9%) and G7.5, (17.6%). In conclusion, glicerol of 5% is recornended for cryopreservation of stallion semen extended with skim milk.