Analisis keragaman genetik enam belas aksesi blewah (Cucumis melo L.) dengan metode random amplified polymorphic DNA (RAPD)

Abstract

Sixteen accession of eilflogenous blewah (Cucumis melo L.) from East Java are subjected to genetics variability analysis on their fruit morphological characters and RAPD assays. Based on five morphological characters including fruit weight, fruit length, flesh thickness, diameter of fruit, and total soluble solid, it is found that the evaluated accessions are separated into two clusters at 3.01 of genetics distance. The first cluster consists of all accessions from Jombang, Lamongan, four accessions from Pasuruan. The second cluster consists of three accessions from Mojokerto, two accessions from Tuban and two accessions from Pasuruan. Accessions from the same origin tend clustered into the same group. Screening of 55 decamer RAPD primers resulted eleven primers are able to amplify the DNA blewah genome, and five out of them are chosen for RAPD analysis. They are OPD9, OP12, OP117, OPN10, and OPN18. RAPD analysis reveals that the accessions are separated into three main clusters at genetics distance of 3.00. The first cluster consists of one accession of No.22 from Mojokerto, the second cluster one accession of No.36 from Lamongan, and the third cluster consists of other fourteen accessions.