Pemanfaatan Bahan Tumbuhan sebagai Biokatalisator dalam Produksi Minyak Sawit Kaya Asam Lemak Omega-3

Abstract

lncorporaton of omega3 polyunsaturated fatty acids (n-3 PUFA) into red palm oil was investigafed by using acido&sis process. Rice bran and Carica papaya latex (CPLI were used as biocatalyst Acidolysis between red palm oil and n-3 PUFA concentrafe in free fatty acid fom was conducted without sohlent, with weight ratio of n-3 PUFA concentrate to palm oil being 1:1. The n-3 PUFA concentrate was prepared from tuna oil by ccrystallization method. The study has demonstrated that rice bran lipase showed higher activdy to incorporate n-3 PUFA into red palm oil than CPL The extent of €PA (eicosapentaenoic acid, C20:5) and DHA (docosahexaeboic acid. C22:6) incorporation were 3.4% and 12.7% with the rice bran lipase, and 1.7% and 3.2% with the CPL. Furlhermore, rice bran from several varifies of paddy (Marnberamo, IR- 64, Merah Munte , and Cirda) produced equal incorporation of €PA and DHA into red palm oil. Rice bran from geminated seed of paddy did not increase the n-3 PUFA incorporation into red palm oil. It means that rice bran in its natural form could be used and act as immobilized lipase. It is easier to remove the rice bran from reaction mixture and reuse the bran for 14 times without decreasing its enzyme activ@.