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Cell Lysis Method Affects Assessment of Microbial Diversity Based on Ribotyping Analysis
(IPB (Bogor Agricultural University), 2008)
The microbial community in Kawah Hujan, Kamojang, West Java, Indonesia, was analyzed using 16S-rRNA-gene-sequencing combining with denaturing-gradient-gel electrophoresis (DGGE) technique. Two different cell lysis methods, ...
The Effect of Mutation at Thr 295 of Saccharomyces cerevisiae eRF1 on Suppression of Nonsense Codons and eRF1 Structur
(IPB (Bogor Agricultural University), 2008)
The termination of translation in Saccharomyces cerevisiae is controlled by two interacting polypeptide chain release factors, eRF1, and eRF3. Two regions in eRF1, at position 281-305 and 411-415, were proposed to be ...
Molecular Dynamics Analysis of Thermostable DNA Pol I ITB-1
(IPB (Bogor Agricultural University), 2007)
One of the thermostable enzymes, which has been widely used in the biotechnological research, is DNA polymerase. The coding sequence of local DNA Pol I gene from a local thermophilic bacterium, namely DNA Pol I ITB-1, has ...
Sixteen-S Ribosomal RNA-Based Analysis of Thermophilic Bacteria in Gedongsongo Hot Spring
(IPB (Bogor Agricultural University), 2007)
Denaturing gradient gel electrophoresis was used to identify the bacterial community at the Gedongsongo (WGS-2) hot spring. The bacterial samples were obtained from both culture dependent and independent strategies. Partial ...