| dc.description.abstract | In 2008, virus-like symptoms of yellowing, interveinal chlorosis, leaf curling and necrotic fleck were observed on greenhouse-tomato plants (Solanum esculentum) in Tochigi Prefecture. The symptomatology and the characteristics of the causal agent such as whitefly transmissibility and particle morphology are similar to those for Tomato infectious chlorosis virus (TICV) and Tomato chlorosis virus (ToCV), species of the genus Crinivirus in the family Closteroviridae. Sequencing of reverse transcription-polymerase chain reaction (RT-PCR) products using the degenerate primers for heat shock protein 70 homolog genes of closteroviruses and specific primers for TICV and ToCV indicated that the virus was ToCV, that has not previously been reported in Japan. In 2008, virus-like symptoms of yellowing, interveinal chlorosis, leaf curling and necrotic fleck were observed on greenhouse-tomato plants (Solanum esculentum) in Tochigi Prefecture. The symptomatology and the characteristics of the causal agent such as whitefly transmissibility and particle morphology are similar to those for Tomato infectious chlorosis virus (TICV) and Tomato chlorosis virus (ToCV), species of the genus Crinivirus in the family Closteroviridae. Sequencing of reverse transcription-polymerase chain reaction (RT-PCR) products using the degenerate primers for heat shock protein 70 homolog genes of closteroviruses and specific primers for TICV and ToCV indicated that the virus was ToCV, that has not previously been reported in Japan. In 2008, virus-like symptoms of yellowing, interveinal chlorosis, leaf curling and necrotic fleck were observed on greenhouse-tomato plants (Solanum esculentum) in Tochigi Prefecture. The symptomatology and the characteristics of the causal agent such as whitefly transmissibility and particle morphology are similar to those for Tomato infectious chlorosis virus (TICV) and Tomato chlorosis virus (ToCV), species of the genus Crinivirus in the family Closteroviridae. Sequencing of reverse transcription-polymerase chain reaction (RT-PCR) products using the degenerate primers for heat shock protein 70 homolog genes of closteroviruses and specific primers for TICV and ToCV indicated that the virus was ToCV, that has not previously been reported in Japan. Keywords Tomato chlorosis virus - Tomato - Crinivirus - Closterovirus - Yellowing disease | id |
