Identification of a Truncated Tn1721-like transposon located on a small plasmid of Escherichia coli isolated from varanus indicus
dc.contributor.author | Waturangi, D.E. | |
dc.contributor.author | S.Schwarz | |
dc.contributor.author | Suwanto, A. | |
dc.contributor.author | C. Kehrenberg | |
dc.contributor.author | W. Erdelen | |
dc.date.accessioned | 2010-06-10T07:09:53Z | |
dc.date.available | 2010-06-10T07:09:53Z | |
dc.date.issued | 2002 | |
dc.identifier.uri | http://repository.ipb.ac.id/handle/123456789/28144 | |
dc.description.abstract | Summary The 9.1 kb plasmid pDEWT1 was isolated from an Escherichia coli strain obtained from the faeces of a free-living lizard (Varanus indicus) in Indonesia. This plasmid mediated tetracycline resistance via a tet gene of hybridization class A. Molecular analysis of a 7755 bp segment of plasmid pDEWT1 including the tetR-tet(A) region and its flanking areas suggested that pDEWT1 harboured a truncated copy of the tet(A)-carrying transposon Tn1721 in which the part responsible for chemotaxis and transposition functions was lost. Analysis of the sequences at the integration site revealed the presence of the 5-bp direct repeat TACTT. The sequences upstream and downstream of the integration site showed striking homology to sequences of a non-coding region detectable on a small cryptic plasmid from Yersinia enterocolitica. | id |
dc.publisher | IPB (Bogor Agricultural University) | |
dc.title | Identification of a Truncated Tn1721-like transposon located on a small plasmid of Escherichia coli isolated from varanus indicus | id |