| dc.description.abstract | We examined 31 blood protein loci, and restriction fragment length profiles of a PCR product of mitochondrial DNA containing the D-loop region using five kinds of restriction endonucleases (HaelII, HinfI, MbolI, MspI, and Sau3AI) in order to quantify the level of genetic variation of longtail macaques, Macacafascicularis. Samples were collected from nine social groups in five localities of West Java, Indonesia. The average heterozygosity per individual (H) was 0.060 and 15.7% of the loci were polymorphic (Ppoly) over all populations in the protein analysis. There was no mtDNA haplotype variation within either social groups or local populations. To the contrary, great diversity was observed among local populations. Both nuclear diversity (measured by NEFS standard genetic distance) and mitochondrial diversity (measured by sequence divergence) showed a significantly positive correlation with geographic distance. There was no significant correlation between these two genetic markers, however. The genetic structure of the population was evaluated in terms of local inbreeding and temporal changes in allele frequency. Differences between nuclear and mitochondrial data are discussed in relation to gender specific migration and lineage sorting. Key Words: Longtail macaques; Blood proteins; mtDNA; Genetic variation; Java Island. | id |
