Screening of Proteolytic Enzymes of Sh.eptomyces sp. Local Strain and Their Characterization

Abstract

Protcmse of two Slreptnmyces sp. strain were chosen Cot characterization betaust of the large cltnr zone surroundlng the colomy In nutrient agar media containing 1% (wlv) akim milk. Extracellular proteam from tbt two Isolatcs SLW 8-1 and 451-3 were cbaractertzed following incubation of the isolate in Nutrlent Brotb media containing sklm milk or chicken feather (1%). Tbe optimum tctivlty of the prottnse SLW 8-1 war at pH 9 and 80 'C, wherean that of the ktratinnsc wms at pH 6.5 and 70 'C. Proteast or straln 451-3 showtd its optimum attlvity at pH 7.5 and 50 *C whereas the kerrtiaast was at pH 8.5 mod 80 T.