dc.contributor.author | Martini S. | |
dc.contributor.author | Nurhayati, Tati | |
dc.contributor.author | Purwanto S.B. | |
dc.contributor.author | Wibawan, I Wayan Teguh | |
dc.date.accessioned | 2010-05-24T03:41:01Z | |
dc.date.available | 2010-05-24T03:41:01Z | |
dc.date.issued | 2005 | |
dc.identifier.uri | http://repository.ipb.ac.id/handle/123456789/24742 | |
dc.description.abstract | A study was carried out to develop a method of enteropathogenic Escherichia coli (EPEC) protease antigen production. In the first trial EPEC strain Kl .1 which was cultured in the LCT-skimmilk- starch medium to produce protease. After purification using centricon this antigen induced non specific polyclonal antibody and cross reacted with the production medium. On the other hand a specific reaction was found from that of the bacteria grown in minimal medium M9 and precipitated with 45% ammonium sulphate. The protease had molecular weight of approximately 58.6 kDa and 37.4 kda with specific protease activity 0.019 1UI mg protein. Key words: enteropathogenic, E. coli, (EPEC), protease enzym, polyclonal antibody | id |
dc.publisher | IPB (Bogor Agricultural University) | |
dc.title | Pengembangan Metode Produksl Antigen Protease Escherichia Coli Enteropatogenik (EPEC) | id |
dc.title.alternative | Development Of Method For Production Of Escherichia Coli Enteropathogenic (EPEC) Antigen | id |