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dc.contributor.authorMartini S.
dc.contributor.authorNurhayati, Tati
dc.contributor.authorPurwanto S.B.
dc.contributor.authorWibawan, I Wayan Teguh
dc.date.accessioned2010-05-24T03:41:01Z
dc.date.available2010-05-24T03:41:01Z
dc.date.issued2005
dc.identifier.urihttp://repository.ipb.ac.id/handle/123456789/24742
dc.description.abstractA study was carried out to develop a method of enteropathogenic Escherichia coli (EPEC) protease antigen production. In the first trial EPEC strain Kl .1 which was cultured in the LCT-skimmilk- starch medium to produce protease. After purification using centricon this antigen induced non specific polyclonal antibody and cross reacted with the production medium. On the other hand a specific reaction was found from that of the bacteria grown in minimal medium M9 and precipitated with 45% ammonium sulphate. The protease had molecular weight of approximately 58.6 kDa and 37.4 kda with specific protease activity 0.019 1UI mg protein. Key words: enteropathogenic, E. coli, (EPEC), protease enzym, polyclonal antibodyid
dc.publisherIPB (Bogor Agricultural University)
dc.titlePengembangan Metode Produksl Antigen Protease Escherichia Coli Enteropatogenik (EPEC)id
dc.title.alternativeDevelopment Of Method For Production Of Escherichia Coli Enteropathogenic (EPEC) Antigenid


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