| dc.description.abstract | The influence of various types of sera i.e. Fetal Calf Serum (FCS), Bovine Post Estrous Serum (BPES) as heterolog sera; and Ewes Fetal Serum (EFS) and Ewes Serzrrn (ES) as homologue sera on in vitro maturation and fertilization of sheep oocytes was investigated s sing -l'iss~ie Culture Medium 1 991-M (TCM 199. Ciibco). Bovinc sera wcre collected I'rom cow at seben days post-estrus, while ewes sera were collected during estrous (ES-HO) and six days post estrous (ES-H6). The result shows that the maturation rate of oocytes cultured in medium supplemented with EFS, ES-HO and ES-H6 at concentrations of 32.9 %, 68.7 %, and 67.6 % respectively is better than those supplemented with FCS and BPES at concentrations of 22.2 % and 28.9 %, respectively. In vitro fertilization rate were significantly higher in medium supplemented with ES-HO and ES-H6 at concentration of 65.4 % and 65.8 % (P < 0.05) than that supplemented with BPES at concentration of 31.7 %. In conclusion, ewes sera could be used in culture medium for promoting both in vitro maturation and fertilization rate of sheep oocytes. Key words: ewes serum, in vitro maturation, in vitr | id |
