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Now showing items 11-18 of 18
Fertilization and Development of Frozen–Thawed Germinal Vesicle Bovine Oocytes by a One-Step Dilution Method in Vitro
(IPB (Bogor Agricultural University), 1996)
The objective of this study was to evaluate in vitro fertilization and cleavage rates of frozen–thawed bovine oocytes at the germinal vesicle (GV) stage. In mouse oocytes, spindle microtubule reorganization after GV breakdown ...
Production of chimeric calves by aggregation of in vitro-fertilized bovine embryos without zonae pellucidae
(IPB (Bogor Agricultural University), 1993)
Bovine embryos produced by in vitro maturation (IVM). fertilization (IVF) and culture (IVC) were used to produce aggregation chimeras. An aggregated chimera was produced by combining bovine IVF embryos (Holstein x Japanese ...
Normal Calves Obtained after Direct Transfer of Vitrified Bovine Embryos Using Ethylene Glycol, Trehalose, and Polyvinylpyrrolidone
(IPB (Bogor Agricultural University), 1996)
In the present study, IVF bovine embryos were vitrified using as the cryoprotectants, ethylene glycol plus trehalose plus the polymer, polyvinylpyrrolidone (PVP). In Experiment I, toxicity of the vitrification solution ...
Pregnancy Rate And Survival In Culture Of In Vitro Fertilized Bovine Embryos Frozen In Various Cryoprotectants And Thawed Using A One-Step System
(IPB (Bogor Agricultural University), 1993)
Bovine oocytes surrounded with compact cumulus cells were cultured for 20 to 22 hours (38.5°C, 5% C02) in modified TCM-199 medium supplemented with 5% superovulated cow serum (SCS) and inseminated by in vitro capacitated ...
Viability Of Bovine Blastocysts Obtained After 7, 8 Or 9 Days Of Culture Following Vitrification And One-Step Rehydration
(IPB (Bogor Agricultural University), 1993)
This study examined the morphological appearance, hatching rates and live:dead cell ratios following vitrification of in vitro produced (IVP) bovine blastocysts. Expanded blastocysts obtained after 7, 8 or 9 d of culture ...
The effect of sperm-oocyte incubation time on in vitro embryo development using sperm from a tetraparental chimeric bull
(IPB (Bogor Agricultural University), 1997)
The present study was designed as 5 X 4 factorial to investigate the effects of using sperm from 5 bulls, and varied sperm-oocyte incubation times (5, 10, 15 and 20 h) on the fertilization, cleavage rates and blastocyst ...
The influence of polyvinylpyrrolidone on freezing of bovine IVF blastocysts following biopsy
(IPB (Bogor Agricultural University), 1995)
A study was conducted to develop a better freezing protocol for in vitro developed biopsled bovine blastocysts. Biopsied blastocysts were exposed to 1.8 M ethylene glycol (EG) + 0.05 M trehalose (T) and different their in ...
Fertility of sperm from a tetraparental Chimeric bull
(Bogor Agriculture University, 1996)
The purpose of the present study was to examine the ability of a tetraparental Chimera in producing IVF embryos. Cumulus oocytes complexes (COCs) were matured in vitro for 22 h. Frozen-thawed sperm of a Chimera (CH), as ...