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Normal Calves Obtained after Direct Transfer of Vitrified Bovine Embryos Using Ethylene Glycol, Trehalose, and Polyvinylpyrrolidone
(IPB (Bogor Agricultural University), 1996)
In the present study, IVF bovine embryos were vitrified using as the cryoprotectants, ethylene glycol plus trehalose plus the polymer, polyvinylpyrrolidone (PVP). In Experiment I, toxicity of the vitrification solution ...
The effect of sperm-oocyte incubation time on in vitro embryo development using sperm from a tetraparental chimeric bull
(IPB (Bogor Agricultural University), 1997)
The present study was designed as 5 X 4 factorial to investigate the effects of using sperm from 5 bulls, and varied sperm-oocyte incubation times (5, 10, 15 and 20 h) on the fertilization, cleavage rates and blastocyst ...
Development of a simple, portable carbon dioxide incubator for in vitro production of bovine embryos
(IPB (Bogor Agricultural University), 1998)
The objective of this study was to develop a simple and portable CO2 incubator using effervescent granules EG.and to examine the effect of negative and positive air pressure for in vitro maturation IVM., fertilization ...
The Influence of Polyvinypyrrolidone on Freezing of Bovine IVF Blastocysts Following Biopsy
(IPB (Bogor Agricultural University), 1996)
A Study was conducted to develop a better freezing protocol for in vitro developed biosied blastocyst. Biopsied blastocysts exposed to 1.8 M ethylene glycol (EG) + 0.05 M trehalose (T) and different concentration (5,10, ...
Fertilization and Development of Frozen–Thawed Germinal Vesicle Bovine Oocytes by a One-Step Dilution Method in Vitro
(IPB (Bogor Agricultural University), 1996)
The objective of this study was to evaluate in vitro fertilization and cleavage rates of frozen–thawed bovine oocytes at the germinal vesicle (GV) stage. In mouse oocytes, spindle microtubule reorganization after GV breakdown ...