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Pregnancy rate and survival in culture of in vitro fertilized bovine embryos frozen in various cryoprotectants and thawed using a one-step system
(IPB (Bogor Agricultural University), 1993)
Bovine oocytes surrounded with compact cumulus cells were cultured for 20-22 h (38.5°C, 5 % C02) in modified TCM -199 supplemented with 5 % superovulated cow serum (SCS) and inseminated by in vitro capacitated sperm. Day ...