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Vitrification of bovine in vitro matured and pronuclear oocites with different vitrification solutions
(IPB (Bogor Agricultural University), 1993)
Vitrification of in vitro matured (IVM) and pronuclear (PN) oocytes was performed using three types of vitrification solutions (VS). IVM oocytes were vitrified with precooled (4°C) VS containing 40% ethylene glycol + 0.3M ...
Fertilization and Development of Frozen–Thawed Germinal Vesicle Bovine Oocytes by a One-Step Dilution Method in Vitro
(IPB (Bogor Agricultural University), 1996)
The objective of this study was to evaluate in vitro fertilization and cleavage rates of frozen–thawed bovine oocytes at the germinal vesicle (GV) stage. In mouse oocytes, spindle microtubule reorganization after GV breakdown ...
Development Of A Simple, Portable Carbon Dioxide Incubator For Production Of Bovine Ivf Embryos
(IPB (Bogor Agricultural University), 1993)
We have developed a simple, economical and portable C02 incubator for production of bovine IVF embryos in the field. The incubator consists of a metallic chamber (29L x 26W x 21 H em); the bottom plate of this chamber is ...
Normal Calves Obtained after Direct Transfer of Vitrified Bovine Embryos Using Ethylene Glycol, Trehalose, and Polyvinylpyrrolidone
(IPB (Bogor Agricultural University), 1996)
In the present study, IVF bovine embryos were vitrified using as the cryoprotectants, ethylene glycol plus trehalose plus the polymer, polyvinylpyrrolidone (PVP). In Experiment I, toxicity of the vitrification solution ...
The effect of sperm-oocyte incubation time on in vitro embryo development using sperm from a tetraparental chimeric bull
(IPB (Bogor Agricultural University), 1997)
The present study was designed as 5 X 4 factorial to investigate the effects of using sperm from 5 bulls, and varied sperm-oocyte incubation times (5, 10, 15 and 20 h) on the fertilization, cleavage rates and blastocyst ...
The influence of polyvinylpyrrolidone on freezing of bovine IVF blastocysts following biopsy
(IPB (Bogor Agricultural University), 1995)
A study was conducted to develop a better freezing protocol for in vitro developed biopsled bovine blastocysts. Biopsied blastocysts were exposed to 1.8 M ethylene glycol (EG) + 0.05 M trehalose (T) and different their in ...
Effect of gas atmosphere on the development of bovine embryos using a simple portable incubator
(Bogor Agriculture University, 1993)
The objective of this study was to examine the effect of gas atmosphere on the development of IVMFC of bovine embryos in a simple portable carbon dioxide incubator under negative and positve pressure of air. Incubation ...
Fertility of sperm from a tetraparental Chimeric bull
(Bogor Agriculture University, 1996)
The purpose of the present study was to examine the ability of a tetraparental Chimera in producing IVF embryos. Cumulus oocytes complexes (COCs) were matured in vitro for 22 h. Frozen-thawed sperm of a Chimera (CH), as ...