Construction Of Specific Primers For Mungbean Yellow Mosaic India Virus Infected Yard Longbean In Java, Indonesia

dc.contributor.author	Nurulita, Sari
dc.contributor.author	Hidayat, Elisabeth Sri Hendrastuti
dc.contributor.author	Muttaqin, Kikin Hamzah
dc.date.accessioned	2023-02-03T12:25:22Z
dc.date.available	2023-02-03T12:25:22Z
dc.date.issued	2022
dc.identifier.uri	http://repository.ipb.ac.id/handle/123456789/116644
dc.description.abstract	Yellow mosaic disease was reported for the first time in Indonesia in 2008. Its infection caused huge significant yield loss. Mungbean yellow mosaic India virus (MYMIV), member of genus Begomovirus was identified as main causal agent. Accurate detection is important as part of disease management strategy. The aim of this study was to construct specific primer pairs for quick and robust detection test. Two sets of specific primer pairs, MYF/MYR and MY1/MY2 were designed in this study to amplify part of Rep – part of CP and part of coat protein only, respectively. In silico and in vitro test showed that these two specific primer pairs specifically amplified MYMIV.	id
dc.language.iso	id	id
dc.title	Construction Of Specific Primers For Mungbean Yellow Mosaic India Virus Infected Yard Longbean In Java, Indonesia	id
dc.type	Technical Report	id
dc.subject.keyword	begomovirus	id
dc.subject.keyword	MYMIV	id
dc.subject.keyword	specific primer	id
dc.subject.keyword	yard long bean	id