| dc.description.abstract | Attempts to rehabilitate degraded natural forests in Indonesia are recently carried out by applying selective
cutting and line planting system. One of the most important aspects in this silvicultural system is the
procurement methods for large number of planting stocks. Shorea johorensis was investigated in this regards
as one of the recommended Shorea species for forest rehabilitaton due to its fast growing character. The
species is usually propagated by three different propagation methods, namely up-rooted seedlings, seeds and
cuttings (stecklings). Genetic consequences due to application of different propagation methods in tlns
species are poorly known and need to be investigated to determine genetic variation and differentiation.
Materials from five origins (populations) in a forest concession holder in Central Borneo, namely: i) uprooted
seedlings, ii) seeds, iii) cuttings, iv) young plantation line and v) natural forest were randomly taken
in the field and subsequently assessed by RAPD technique using three previously tested random primers of
OPO-11, OPO-13 and OPO-16. Results showed that among 5 populations investigated, natural tree
populations showed the lnghest levels of genetic variation with mean values na = 1.2593, ne = 1.2070, PPL =
25.93% and He = 0.1109. Cutting populations showed the lowest levels of genetic variation with mean values
na = 1.1111, ne = 1.0773, PPL = 1l.11 % and He = 0.0445. Meanwhile, according to the propagation
methods, up-rooted seedling population revealed the highest levels of genetic variation with mean values na
= 1.2222, ne = l.1613, PPL = 22.22% and He = 0.0886. Values of ne and He in natural forest were higher (ne
= 1.2070 and He = 0.1109) than those of young plantation line (ne = l.1609 and He = 0.0896). The closest
genetic distance was observed between population of seeds and cuttings, namely 0.0590. It was found that a
particular procedure to propagate planting stocks at large scale in this company, i.e. cutting propagation
method, tended to reduce genetic variation. | id |
