Genetic Diversity of Cytochrome b of Crowned-pigeons (Goura sp.)

Abstract

Crowned-pigeon (Goura sp.) was one of endemic animal in Indonesia with “vulnerable" status which spread at Papua New Guinea and Irian Jaya Island. Conservation effort to this species will succeed if morphology characteristic and genetic diversity be known. The main purpose of this research is to analyze nucleotide variability of cytochrome b partial using Polymerase Chain Reaction method. The PCR product amplified by primer M101 and M102, then multiple aligment using Genetyx-Win 3.0™ and Clustal-X™, furthermore analyzed with MEGA 3.0™. The result of analyse from 382 nucleotide of cytochrome b partial encoding 127 amino acid. Findings 42 sites of nucleotide variable with average of transitional substitutions 0.05 and average transversions substitutions is 0.01. Value non-synonymous mutations is 7.08% (10 amino acid sites) whereas synonymus mutations is 23.62% (30 amino acid sites). Value of genetic distance of nucleotide cytochrome b (method p-distance) range from 0.26% - 7.07% with average 5.26%. Filogenetic reconstruction using Neighbor Joining method indicate that G.cristata were closer to G.scheepmakeri compare with G.victoria.